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LF also enhances the host cells antiviral defenses, blocking viral replication ( 4.2.2 Mechanism of action in dry eye disease LFs antioxidant properties on the ocular surface, where it chelates free iron and prevents the formation of harmful hydroxyl radicals, thus protecting CECs from oxidative stress (Pastori et al., 2015)

ATP Measurement An enhanced ATP assay kit (Beyotime, S0027, China) was used to measure total SNr tissue ATP levels according to the instructions of the manufacturer

Cells were treated with or without 300 ng/ml doxycycline, 5 M A, 350 M buthionine sulfoximine (BSO), 5 M dimethyl fumarate (DMF), and 0.1 M ferrostatin-1 (Fer-1), as indicated

Bulgaria J