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It can be synthesized using different petroleum-based chemical methods, including (i) nitric acid oxidation of 2-cyanocylopentanone, (ii) condensation of acrylonitrile with ethyl malonate, (iii) ozonation of cyclopentane and permanganate cleavage of ozonide, and (iv) oxidation of pentamethylene glycol using nitrogen tetroxide 3,4,5

Beads were washed four times with IP wash buffer (50 mM TrisHCl (pH 7.4), 150 mM NaCl, 0.1% (vol/vol) IGEPAL CA-630), then thrice with IP wash buffer lacking IGEPAL CA-630

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