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HEK293 cells were lysed in CSK Buffer (10 mM Pipes pH 7.0, 300 mM sucrose, 100 mM NaCl, 3 mM MgCl 2 ) supplemented with 0.1% Triton X-100, Roche Complete EDTA-free protease inhibitor tablet (#11 873 580 001), and Sigma phosphatase inhibitor cocktail 3 (#P0044) for 30 min on ice

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