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To have the optimal and most realistic conditions, we applied the same technical criteria (same antibody, same dilution, and same cutoff) as in the study of Prieto-Granada et al

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Postnatal cortical neuron isolation and culture Postnatal cortical neurons were isolated following a protocol adapted from Brewer and Torricelli (2007) and the Miltenyi Biotec MACS protocol for the isolation and cultivation of neurons from adult mouse brain