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Vitamin B12 injections are given intramuscularly for those with a B12 deficiency related to age, pernicious anaemia, or low levels caused by a vegan or restrictive diet without supplementation

For immunohistochemistry (IHC) labeling, heat-mediated epitope retrieval was performed in 0.01 m sodium citrate buffer, pH 6, containing 0.05% Tween-20, which was heated in a microwave oven to 95C for 30 min

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