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Cell lysates were collected in cell lysis buffer (20 mM Tris, 0.5 mM dithiothreitol, 150 mM NaCl, 0.5% Triton X-100, 2 g/mL pepstatin, 2 g/mL bestatin, 2 g/mL leupeptin, 4 mM phenylmethylsulfonyl fluoride, 10 g/mL aprotinin, 1 mM tetra-sodium pyrophosphate decahydrate, 10 mM -glycerophosphate, 1 mM sodium orthovanadate, 1 M sodium fluoride, pH 7.5)

When you have impaired thyroid function, TSH is underutilized, so the number gets higher

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Moreover, the variation in the magnitude of rescue between CT and BD suggests underlying differences in inflammasome and redox regulation and mitochondrial dysfunction between the two groups, warranting further investigation into their distinct inflammatory and mitochondrial profiles

Strhle A et al