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The inclusion of 0.9% benzyl alcohol provides antimicrobial preservation, helping maintain solution integrity following repeated vial access when appropriate aseptic laboratory techniques are utilized

Each coverslip was washed three times with PBS, blocked with blocking solution (1x PBS, 10 % normal goat serum (NGS) and 0.3 % Triton X-100) for 30 min at RT and incubated with primary antibodies (anti-GFP (rabbit polyclonal, used at 1:1000 in IF, Abcam, Cat# ab6556, RRID:AB_305564), anti vGLUT1 (guinea pig polyclonal, used at 1:500 in IF, Synaptic Systems, Cat# 135 304, RRID:AB_887878)) diluted in blocking solution for 2 h at RT

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Wolfe GI, Ward ES, de Haard H, Ulrichts P, Mozaffar T, Pasnoor M, et al

For most people, arm soreness after a vaccination is normal and fades within a few days