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Store lyophilized material at 4 F (20 C) or below Allow vial to reach room temperature before opening Protect from light, heat, and humidity Use sterile laboratory equipment during preparation Avoid repeated freezethaw cycles Label reconstituted samples with preparation date and concentration These practices support consistent experimental outcomes and reduce variability across assays

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A recent study shows that IL-22 expression is significantly increased in the liver of female mice with APAP-induced hepatotoxicity, indicating the necessity to consider gender-dependent effects when using mice to establish a pathophysiological model of IL-22 [205]

doi: 10.1038/ni.1645 59 GeFWangFYanXLiZWangX