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Burns JM, et al

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Protein Extraction, Immunoblotting, and Proteomic Analysis Enteroid monolayers were lysed in cold lysis buffer (60 mM HEPES pH 7.4, 150 mM KCl, 5 mM Na3EDTA, 5 mM EGTA, 1 mM Na3VO4, 50 mM NaF, 2% SDS) supplemented with 1:100 of protease inhibitor cocktail (P8340, Millipore Sigma)