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Membranes were then incubated overnight at 4C with the following primary antibodies: NINJ1 (1:1000, Invitrogen, PA5-95755), GAPDH (1:1000, Cell Signaling, #97166), PANK1 (1:1000, Cell Signaling, #23887), xCT (1:1000, Cell Signaling, #12691), V5 tag (1:1000, Cell Signaling, #13202), and -tubulin (1:1000, Cell Signaling, #2128)

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Here, we deciphered the role of the redox homeostasis in Wobbler spinal cord and dissociated motor neuronal enriched cell culture on the formation of toxic DNA double strand breaks (DNBs) as well as the impact of ROS scavengers on the prevention of integrity of Wobbler motor neuron DNA