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Dual luciferase reporter assay For the luciferase assay, 293 T cells were cultured in 24-well plates at 70 to 80% confluency and co-transfected with the plasmids encoding Ppara -wild, Ppara -mut, Pparg -wild, or Ppard- wild, along with the PPRE plasmid and a control vector (Renilla luciferase), using Lipofectamine 3000 Reagent (Invitrogen, L3000150) according to the manufacturers protocols

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In the US, the legal limit for BAC when driving is 0.08%