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Description

Current data are limited to preclinical research models, where MP-2 is used as a tool to investigate immune regulation, cellular recovery mechanisms, and tumor-associated immunosuppression

2010;299:G23G31

After confirming that the concentration of NADPH in our lysates was within the linear detection range of the assay (Fig 3A), we treated LPS-stimulated (6 h) RAW264.7 Ms with 2-DG and diamide and quantified NADPH kinetics by obtaining lysates at 5-minute intervals for a total of 25 minutes (Fig 3B)