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The eluted protein was concentrated by a size-exclusion spin column and exchanged into BRB80 buffer (80 mM HEPES, 1 mM MgCl 2 , and 1 mM EGTA, pH 6.8 by KOH) with 10% glycerol, 50 M ATP and 5 mM DTT

An amino acid called cysteine is a particularly important amino acid that is involved in glutathione synthesis

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