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In addition, presence of high concentrations of polyunsaturated fatty acids in the brain enhances their peroxidation and generation of toxic products which further causes degeneration of the cells harboring them or in vicinity 22

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In brief, after removal of the perichondrium, dissected growth plates were digested using 2 mg ml 1 collagenase type II in minimum essential medium alpha (MEM) GLUTAMAX supplemented with 100 units per ml penicillin, 50 g ml 1 streptomycin and 10% FBS (all from Gibco, ThermoFisher Scientific) for 3 h at 37 C

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