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Pitfalls in Testing for B12 Deficiency How do you know if you have a B12 deficiency

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[35] Although the mechanism is not very clear, the polyglutamylation of methotrexate and dihydrofolate in malignant cells is considered to play an important role in the selective reactivation of dihydrofolate reductase by folinic acid in normal cells

The HTS Core has been supported in part by the State of Colorado Office of Economic Development and the Colorado Clinical and Translational Sciences Institute (NIH Grant UL1TR001082), the University of Colorado Anschutz Medical Campus

Enzymatic assays To assay the activities of Cp1A and MfaA, 100-l reactions were performed at 30 C for 3 h in 50 mM sodium phosphate buffer (pH 8.0) containing 0.2 mM FeSO 4 , 2 mM KG, 2 mM ascorbate, 1 mM substrate and 10 M Cp1A or MfaA