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The increase in biotin may be due to fluctuations in diet, seasonal changes, or the plant-based extract which was used in the placebo (not the supplement) to give it a "vitamin-like" flavor

The most common example comes from the deficiency of folate and/or vitamin B 12, which would disturb the methylation-dependent pathways by incapacitating a source of methyl groups, a phenomenon that has been linked to mental retardation, psychiatric disorders, myelopathy and neurological diseases [9, 18]

As expected, since MTT and CTG assays measure cellular metabolism, the CC 50 values of both compounds were somehow lower in the various cell types as compared to growth curve assessment