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Therefore, in this study, an oxidative stress-induced senescence model in HaCaT cells was established using H 2 O 2

88 In recent years, studies have revealed that UA activates the TLR4-NLRP3 inflammatory complex, which is a multi-protein complex that plays a pivotal role in initiating the innate immune response to various danger signals, including MSU crystals

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However, risks include surgical complications, hemorrhage, infection, and neuropsychiatric side effects, necessitating careful patient selection and multidisciplinary evaluation (Cury et al., 2022

The pentose phosphate pathway (PPP) is the major supply of NADPH in cells and so cells were incubated with 6-amino nicotinamide, a known inhibitor of the PPP enzyme 6-phosphogluconate dehydrogenase