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Herein, the simulation system was amended with an explicit TIP4P (transferable intermolecular potential-4 point) solvent model, as an orthorhombic box (10 10 10 buffer) followed by the addition of 0.15 M salt to provide a physiological environment using the System Builder tool

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This is because every case happens in the context of complicated factors such as duration of illness, the bodys own immune response, and the presence of co-infections or other co-morbidities