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Densitometry was evaluated using ImageJ software

The frequency of Vitamin B12 injections can vary based on individual needs, health status, and the severity of the deficiency

[DOI] [Google Scholar] Devesa, A

J Neurosci 36:1075010758

The membranes were sequentially probed with the primary antibodies (1:5000) at 4 C overnight and corresponding horseradish peroxidase-conjugated secondary antibodies (1:10,000, Pierce at room temperature for 1 h)