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This binding site is deeply buried within the core of the transporter protein and is structurally formed by amino acids located on Transmembrane Helices (TMHs) 1, 3, 6, and 8 (Beuming et al., 2008

EtG was 78% sensitive and 75% specific for past 3-day reported use, 68% sensitive and 88% specific for detectable Peth, and 66% sensitive and 59% specific for reported AUD

This effect is likely to be mediated through several pathways: a) Upregulation of antioxidant enzymes: The significant increase in catalase levels observed in the IF group suggests that IF enhances endogenous antioxidant defenses, as catalase is a key enzyme in detoxifying hydrogen peroxide and mitigating oxidative stress