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Western blot analysis HaCaT cells (Anweisci, Shanghai, China) were cultured in petri dishes until the cell density reached 80%, and then the cells were exposed to 100 g/mL TAT-CcSOD1 for varying durations of 0, 15, 30, 60, and 120 min

Moreover, ROS can directly target ETC complexes, especially complex I and complex III, causing electron leakage and amplifying ROS production, which further intensifies oxidative stress and mitochondrial dysfunction [26]

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But actually there's more common causes of muscle spasms and often it has to do with electrolytes

(2001) Endothelial transcytosis of myeloperoxidase confers specificity to vascular ECM proteins as targets of tyrosine nitration, J