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Data were statistically processed by a two-way analysis of variance using Statgraphics Program (Statistical Graphics Corporation, 1991)

Oxidative stress plays a significant role in MS pathogenesis by inducing inflammation, demyelination, and neurodegeneration

Mechanism of Action: A cholinergic parasympathomimetic agent exerting a broad spectrum of pharmacologic effects with predominant muscarinic action Indications: (Salagen ) resulting from salivary gland hypofunction caused by: Treatment of dry mouth Radiotherapy for cancer of the head and neck Sjgrens syndrome, an autoimmune disorder where immune cells attack and destroy the glands that produce tears and saliva Treatment of glaucoma (Open-Angle & Acute Angle-Closure)(Isopto Carpine ) eye drops containing pilocarpine cause contraction of the ciliary muscle which opens the trabecular meshwork spaces to increase the outflow of aqueous humor through the canal of Schlemm , thereby lowering intra-ocular pressure

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Total RNA was extracted using an acid guanidinium thiocyanatephenolchloroform method, purified with an RNA extraction kit, and treated with DNase to remove genomic DNA contamination