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In brief, cDNA was generated using M-MLV reverse transcriptase (Thermo Fisher) from RNA samples isolated from the cultures, and then incubated with Fast SYBR Green Master Mix (Applied Biosystems)

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10.1016/0006-2952(87)90130-4 Biochem

Besides, IH could promote neuronal apoptosis in normal mice and diabetes mice models, the latter had higher apoptosis levels and could be modulated by high mobility group box-1 protein (HMGB1)/toll-like receptor 4 (TLR4) signal [53]