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In addition, evaluating inhibitory tonus as a measure of repressed plasticity in the post-injured brain, concomitant changes in the excitatory input may occur, making it difficult to estimate changes in the overall excitatory/inhibitory balance of the neural circuits undergoing rearrangement

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Gsh1 catalyzes the formation of -glutamylcysteine (-GC), which is subsequently conjugated to glycine by Gsh2 to form GSH

PMID 33160102

For orthotopic cell transplantation experiments, 6-week-old C57BL/6 male mice were injected with 100,000 cells (p53-1 or N1IC-1, passage 10, isolated from littermates) resuspended in 2 L PBS at the following coordinates (with bregma as reference): 2.5 mm lateral, 2 mm rostral, and 2.5 mm deep, and at a flow rate of 0.25 L/min