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The nanobody (Nb) 2Rs15d 53 with an N-terminal Gly-Pap-GG tag was expressed in the cytoplasm of NEBExpress(lowRF1) and purified as described above for AmpC

Beyond macrophages and neutrophils, other immune cells such as dendritic cells and T and B lymphocytes may also participate in ferroptosis-related immune regulation in periodontitis, collectively forming a multilayered and interconnected immune-ferroptosis network

Samples were then either snap-frozen in liquid nitrogen and stored at 20 C before analysis, or immediately injected to the mass spectrometer (Extended Data Fig

-glutamylcysteine synthetase in higher plants: catalytic properties and subcellular localization

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