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To facilitate the clinical integration of emerging biomarkers in MASLD diagnosis and management, a flowchart summarizing the proposed stepwise implementation ranging from initial clinical assessment to biomarker-guided monitoring is presented in Figure 4

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A GC-MS-based metabolomics analysis further identified 716 metabolites in the human body, with certain metabolites exhibiting age-related upregulation or downregulation, significantly affecting human physiology

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Another way of considering the chemical basis of these complementary approaches is to note that in procedure A, we leverage the fact that in solution, the deprotonated terminal perthiolate and perthiolate-like sulfur atoms in polysulfides have nucleophilic chemical properties and thus react with IAM/IAM-TPP