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Thereafter, horseradish peroxidase (HRP)-conjugated goat anti-human IgG (Jackson Immunoresearch, West Grove, PA) was diluted in PBS+1%BSA to a final dilution of 1:1,000, and 50 L of this dilution was added to each well in the microtiter plates

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MTHFR Gene Variants with Hyperhomocysteinemia and Cardiovascular Diseases Elevated Hcy levels are considered an independent risk factor for the development of CVD, which encompasses CAD, MI, IS, hypertension, and thrombosis [84,85]

Aberrant expression or hyperactivation of zinc transporters would also contribute to tumor resistance, which could be a malprognostic factor for cancer patients

150 mM NaCl