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Samples were resolved on 10% SDS-polyacrylamide gels and DNP-derivatized proteins were identified by Western blot analysis using an anti-DNP antibody and an appropriate horseradish peroxidase-conjugated secondary antibody

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CD4 + EM1 + T cells are known to home to tissues and produce IFN- within hours of stimulation 20

For comparison, in our studies, both Phase-I and Phase-II HSB were fed at satiety and the diet contained 0.56% cysteine [13, 14]

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