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Figure 4 Future directions A major hurdle in dissecting the functions of individual Wnt ligands and Frizzled receptors is their redundancy

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Inject the solvent into the peptide vial along the inside glass wall rather than directly onto the lyophilized powder, then swirl gently in a circular motion until the powder dissolves completely do not shake, vortex, or invert vigorously, as mechanical agitation can denature peptide secondary structure