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Coverslips were mounted in Fluoromount-G (SouthernBiotech) and cured for 24 h before imaging

Erythrocytes must be washed and lysed, and the assay must be performed immediately after blood samples are drawn because FAD is a labile compound (Shenkin and Roberts, 2016)

Thus, this study opened the door to a new strategy for the generation of PROTACs, as well as the generation of PROTACs with potential for the treatment of neurodegenerative diseases, given the involvement of Sirt2 in Parkinsons disease (Table 12) 169