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Using fluorescence polarization binding assays, we found that SERF2 binds with sub-micromolar affinities to various RNA sequences that are known to fold into rG4 structures 3,44 , including polyG, (GGGA) 4 , (GGA) 7 , (G4C2) 4 and (AGG) 5

After H 2 O 2 incubation, gels are stained with ferric chloride and potassium ferricyanide, forming Prussian blue precipitates except in regions of CAT activity, which appear as clear bands against stained backgrounds (Pezzoni et al., 2018)