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For single sgRNA cloning, the oligonucleotides containing 20 bp protospacer and overhang were obtained from Integrated DNA Technologies and cloned into the Bsm BI-v2 (New England Biolabs, catalogue no

Kubatka P, Kapinov A, Kello M et al (2016) Fruit peel polyphenols demonstrate substantial anti-tumour effects in the model of breast cancer

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elegans is associated with piRNAs, 22G-RNAs, and histone H3K9me3 and H3K27me3 marks 11,35,86,114

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