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The gRNA target sites were designed using online tools ( The primers used for amplification of gRNA templates were as follows: T7-akt1-gRNA-F: 5-TAATACGACTCACTATAGGGACAATGATTACGGTCGTGGTTTTAGAGCTAGAAATAGC-3

Detection of the circulating antigens CAA and CCA in a group of Dutch travellers with acute schistosomiasis

Start with the guidelines in this article but closely observe how you feel and adjust accordingly