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The pellet was resuspend in 150 l complete lysis buffer and incubated on ice for 30 min

for 10 min, 25 L of agonist was dispensed into the well using a FlexStation III microplate reader (Molecular Devices), and the intracellular calcium change was recorded at an excitation wavelength of 485 nm and an emission wavelength of 525 nm

For a vial with 20 draws, you lose approximately 0.14-0.24mL to dead volume

IGF-1 LR3, also known as Long-Arg3-IGF-I, is a synthetic peptide similar to Insulin-like Growth Factor 1 (IGF-1)

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