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After a 4 min wash the pre-column was switched (Valco 10 port UPLC valve, Valco, Houston, TX) into line with a fritless nano column (75 m x ~ 15 cm) containing C18AQ media (1.9 L, 120 Dr Maisch, Ammerbuch-Entringen Germany)

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This number, 0.4368 nM, is the average E 2 over the menstrual cycle computed from the data in Panel A of Figure 2

10.1152/ajpcell.00239.2023 146 ParkS.MoriR.ShimokawaI

Other Ingredients: Microcrystalline Cellulose, Hypromellose, Vegetable Magnesium Stearate, Silicon Dioxide