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Then, the samples were vigorously vortexed, incubated at RT for 20 min and centrifuged (4 C, 12,000 g , 20 min)

Lancet Diabetes Endocrinol 11(4):282298 Wyne KL, Nair L, Schneiderman CP, Pinsky B, Flores OA, Guo D, Barger B, Tessnow AH (2023) Hypothyroidism prevalence in the United States: a retrospective study combining national health and nutrition examination survey and claims data

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LDL (commonly referred to as the bad cholesterol) can transport cholesterol to arterial walls, contributing to the development of atherosclerosis (hardening and narrowing of arteries) and cardiovascular disease