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Ascorbic acid was shown to cause Dev R regulation by an extra process

The two supernatants were combined, the pH was adjusted to 5 with potassium hydroxide, a 10 L aliquot was collected for LC-MS analysis and the sample was frozen-dried

This process promotes the sequestration of calcium ions in the sarcoplasmic reticulum while reducing their concentration in the cytosol, which impacts phosphorylation and consequently results in smooth muscle relaxation (Fig