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The concentration of total protein was determined by modified Bradford reagent (Sangon Biotech, China) using bovine serum albumin as the standard

It's three separate problems compounding on top of each other and understanding all three helps explain why this condition is so hard to catch before it causes serious damage

The authors would like to thank Associate Professor Cui Dan (Department of Pathology, Yamaguchi University Graduate School of Medicine) for their skilled technical support and helpful suggestions

Enhanced ROS formation as a consequence of increased oncogenic signaling and a compensatory increase of cellular antioxidant defense have been described by others ( Figure 1C )

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