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It also inhibits the production of pro-inflammatory cytokines (TNF-, IL-6, excess TGF-) while stimulating anti-inflammatory cytokines (IL-10)

doi:10.1038/s42255-023-00916-6

The experiments were conducted in triplicate, and the data are presented as mean standard deviation

How IV Ozone Therapy Works (Major Autohaemotherapy) The gold-standard method used in clinics is Major Autohaemotherapy (MAH): A small volume of blood is drawn It is exposed to a precise oxygenozone mixture The treated blood is re-infused into the body This process creates a controlled, temporary oxidative stimulus, which: Activates antioxidant enzymes Improves oxygen delivery to tissues Increases cellular energy (ATP production) Stimulates immune signalling pathways Clinical literature describes this as a form of controlled oxidative stress that triggers beneficial biological responses when administered correctly

To assess whether these transcriptomic changes were specific to the actions of DSF, we conducted expression profile overlap analysis of data obtained from JE2 cultures treated with its primary metabolite DDTC at 4x MIC (Fig