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This cascade results in the cleavage of Gasdermin D, leading to the formation of membrane pores that may contribute to cell lysis and the release of the proinflammatory cytokines interleukin (IL)-1 and IL-18

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Six hours after transfection the medium (DMEM, 10% FBS, 2mM L-glutamine without antibiotics) was replaced, and cells incubated for 18 hours before the medium was harvested for viral infection of recipient cells