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A typical reaction would take place at 37 C and include a reaction mixture containing d-PhgAT or HeT (0.018 mg/mL or 0.05 mg/mL respectively), sodium pyruvate (2.5 mM), (s)-methylbenzylamine (2.5 mM), PLP (0.1 mM) in potassium phosphate buffer (50 mM, pH8) and DMSO (0.25%v/v) in a final reaction volume of 1 mL 40,41