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For instance, it has been reported that activation of Bdnf transcription peaked at 1 h after depolarization 21 whereas luciferase activity increased gradually and peaked at approximately 7 h after depolarization 20

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A plot of the concentration versus the time was then generated in able to find the degradation kinetic constant and calculate the half life Microsomal metabolism was also examined by the research team using the liver microsomes from male rats and an NADPH-regenerating system prepared by adding 1.7 mg/ml NADP, 7.8 mg/ml glucose 6-phosphate, and 6 U/ml glucose-6-phosphate dehydrogenase to 10 ml of 2% sodium bicarbonate

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