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Inhibition of the first rate-limiting enzyme of one-carbon metabolism (PHGDH) has been shown to promote cell growth arrest, a phenomenon that occurs as a result of an accumulation of intracellular reactive oxygen species (ROS) following the inhibition of glutamine metabolism

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To quantify the amount of CO, the ratio of carboxy-haemoglobin after haemoglobin addition was measured [21]

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At the conclusion of the 28-day treatment period, the efficacy of each treatment was assessed and mice were humanely euthanized using CO 2 gas