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Recombinant protein purification and in vitro kinase assay Full-length human G6PD (wild-type, 2YA or 2YF mutant) sequences were cloned into the pGEX-4T1 vector and expressed as the GST-tagged fusion proteins in E

10.1038/nature02661 165 LuJ.XuY.HuW.GaoY.NiX.ShengH.et al

doi: 10.1016/j.bbagen.2012.11.020 16

Full wavelength absorption of DOX&IR780@PEG-PCL-SS NPs shows that the nanoparticles have two absorption peaks at 497 nm and 780 nm, which are the characteristics of doxorubicin and IR780, respectively

or without these intermediate steps but also before and / or after pre-treatment and / or post-treatment steps such as acid washing, Q levels, alkaline leaching ,