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Determination of vesicle size, polydispersity index, and zeta-potential The average particle size, polydispersity index (PDI), and zeta potential of the fabricated ivermectin nanoformulations were determined using a Malvern Zetasizer Nano series ZS instrument (Malvern Instruments, Malvern, UK) after appropriate dilution at room temperature 37

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Similar approaches in other species have confirmed that SPDS is a robust engineering target for boosting spermidine-linked stress resilience 87,88

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