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Cells were lysed by gentle pipetting and incubated on a rotating shaker at 4 C for 15 min

McGraw Hill Book Co., New York Lutts S, Kinet J, Bouharmont J (1995) Changes in plant response to NaCl during development of rice ( Oryza sativa L.) varieties differing in salinity resistance

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First-line obesity treatments include lifestyle intervention (e.g., low-calorie diets) and behavioral modification therapies 4,5 since approved obesity medications have limited efficacy and commonly have side effects 6