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After 5% GSA blocking at room temperature for 1 h, the cells were incubated sequentially with the specific primary antibodies overnight at 4 C and fluorescent secondary antibodies in 5% bovine serum albumin for 1 h at 37 C

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In 2012, the second-generation AR antagonist enzalutamide (Figure 6) (MDV3100) was approved by the FDA for use in prostate cancer 159,160,161

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