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IL-2-neutralizing antibodies, 25 g ml 1 S4B6 (BioXCell) and 25 g ml 1 JES6.1 (BioXCell) were directly added after activation in non-arrested conditions or after removal of the cell cycle inhibitor in released conditions

However, by using stable isotope labeling in isolated perfused mouse hearts, recent models on the mechanism and origin of ischemic succinate accumulation proposed that succinate primarily derives from the existing TCA cycle metabolite pool and canonical TCA cycle activity, with only a minor contribution to ischemic succinate of mitochondrial complex II reversal (reducing fumarate to succinate) and aminotransferase anaplerosis 55 (originating fumarate from aspartate)

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doi:10.1046/j.1524-475X.1994.20406.x 9