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Smedfors G, Olson L, Karlsson T

doi: 10.1111/j.1471-4159.1971.tb11980.x

The phagocytized non-photosynthetic bacteria (symbionts) over eons evolved into mitochondria while the engulfed photosynthesizing bacteria became chloroplasts (known as the well-established endosymbiotic theory) of the early eukaryotes and during this process the melatonin-synthesizing capacity of the bacteria-derived organelles, both chloroplasts and mitochondria, was retained ( Figure 2 )

Then, membrane pellets were resuspended in a Dounce homogenizer in lysis buffer