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Not to mention that any injection has a slight risk of infection

Studies examining fibroblast and endothelial cell systems show increased phosphorylation of focal adhesion kinase and paxillin, two proteins central to cytoskeletal organization and cell adhesion signaling

Let's look at how that translates to dosing with a U-100 syringe: 5mg is in 100 units (1 mL) 1mg is in 20 units (0.2 mL) 0.5mg is in 10 units (0.1 mL) As you can see, a 1mg dose now requires twice the volume (20 units instead of 10)